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Literature summary extracted from

  • Pardo, J.M.; Malpartida, F.; Rico, M.; Jimenez, A.
    Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus - the producing organism (1985), J. Gen. Microbiol., 131, 1289-1298.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.119 hygromycin B above 0.01 mM, substrate inhibition Streptomyces hygroscopicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.119 additional information
-
 additional information
-
 Streptomyces hygroscopicus
2.7.1.119 0.0015
-
 hygromycin B pH 7.5, 30°C Streptomyces hygroscopicus
2.7.1.119 0.076
-
 ATP pH 7.5, 30°C Streptomyces hygroscopicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.119 Mg2+
-
 Streptomyces hygroscopicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.119 42000
-
 gel filtration Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.119 ATP + hygromycin B Streptomyces hygroscopicus resistance to hygromycin B also occurs in Escherichia coli, where a plasmid encodes a hygromycin B phosphotransferase activity that produces 4-phosphoryl-hygromycin B ADP + 7''-O-phosphohygromycin B
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.119 Streptomyces hygroscopicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.119 partial Streptomyces hygroscopicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.119 0.0003
-
-
 Streptomyces hygroscopicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.119 ATP + hygromycin B
-
 Streptomyces hygroscopicus ADP + 7''-O-phosphohygromycin B
-
 ?
2.7.1.119 ATP + hygromycin B resistance to hygromycin B also occurs in Escherichia coli, where a plasmid encodes a hygromycin B phosphotransferase activity that produces 4-phosphoryl-hygromycin B Streptomyces hygroscopicus ADP + 7''-O-phosphohygromycin B
-
 ?